Triazolopyridine compounds as PIM kinase inhibitors

ABSTRACT

Compounds of Formula (I): I in which B, R 1 , R 1a , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 10  and R 11  have the meanings given in the specification, are receptor tyrosine inhibitors useful in the treatment of diseases mediated by PIM-1 and/or PIM-2 and/or PIM-3 kinases.

The present invention relates to novel compounds, to pharmaceuticalcompositions comprising the compounds, to a process for making thecompounds and to the use of the compounds in therapy. More particularly,it relates to certain triazolopyridine compounds useful in the treatmentand prevention of diseases which can be treated with a PIM kinaseinhibitor, including diseases mediated by PIM kinases. Particularcompounds of this invention have been found to be inhibitors of PIM-1and/or PIM-2 and/or PIM-3.

Protein kinases constitute a family of structurally related enzymes thatare responsible for the control of a vast array of cellular processes.

The PIM kinase sub-family consists of three distinct serine/threonineprotein kinase isoforms (PIM-1, -2 and -3) belonging to thecalmodulin-dependent protein kinase-related (CAMK) group. PIM-2 andPIM-3 are respectively 58% and 69% identical to PIM-1 at the amino acidlevel.

The over-expression of PIM-1 has been reported in various humanlymphomas and acute leukemias (Amson, R. et al, Proc. Natl. Acad. Sci.U.S.A., 1989, 86: 8857-8861). PIM-1 has been shown to synergize withc-Myc to drive lymphomagenesis (Breuer M., et. al., Nature, 1989, 340;61-63), and plays an important role in cytokine signaling in T-celldevelopment (Schmidt, T., et. al., EMBO J, 1998, 17:5349-5359). Inaddition, there is evidence that PIM-1 is over-expressed in prostaticneoplasia and human prostate cancer (Valdman, A. et al, The Prostate,2004, 60: 367-371; Cibull, T. L. et al, J. Clin. Pathol., 2006, 59:285-288) and may serve as a useful biomarker in identification ofprostate cancer (Dhanasekaran, S. M. et al, Nature, 2001, 412(13):822-826). PIM-1 has been shown to be critical for IL-6 mediatedproliferation of hematopoietic cells (Hirano, T., et. al. Oncogene 2000,19:2548-2556), as well as STAT3 mediated cell cycle progression(Shirogane, T., et al., Immunity 1999, 11:709.

Recently, it has been discovered that PIM-1 is up-regulated by Flt-3 andmay play an important role in Flt-3 mediated cell survival (Kim, K. T.et al Neoplasia, 2005, 105(4): 1759-1767). Since Flt-3 itself isimplicated in leukemias like AML, additional knockdown of PIM-1 may be auseful approach to treating leukemias driven by Flt-3 or variousmutations. Accordingly, PIM-1 inhibitors may be useful as therapeuticagents for a variety of cancers such as hematological cancers.

PIM-2 is a highly conserved serine/threonine kinase involved in cellproliferation and the prevention of apoptosis (Baytel et al., Biochim.Biophys. Acta Gene Struct. Expr. 1442: 274 (1998)). PIM-2 is upregulatedin AML, CLL, and possibly in prostate cancer.

PIM-3 is a proto-oncogene identified in pancreatic liver and coloncancers, and is an apoptotic regulator (Popivanova, B., et al., CancerSci., 98(3): 321 (2007)).

Based upon the direct involvement of the PIM kinases in a wide varietyof cancers downstream of STAT3/5 activation, it is expected thatinhibition of the PIM kinases will result in inhibition of proliferationand survival of multiple cancer cell types. This would then be expectedto provide a therapeutic benefit to cancer patients with a variety ofcancers (both solid tumor and hematologic settings), as well as otherconditions that are mediated by PIM kinase signaling.

In addition to the malignant cells detailed above, PIM kinases are alsoexpressed in hematopoietically-derived cell lines andhematopoietically-derived primary cells including cells of the immunesystem such as B cells, T cells, monocytes, macrophages, eosinophils,basophils, and dendritic cells. Expression of PIM kinases can beinduced, for example, by cytokines which utilize Jak/Stat signaling,such as IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-12, IL-15, GM-CSF,IFNα, IFNγ, erythropoietin, thrombopoietin, and prolactin, and thegeneration, differentiation, maintenance and activation ofhematopoietically-derived cells is dependent on these cytokinesMoreover, PIM proteins have been shown to be required for the efficientproliferation of peripheral T cells mediated by T-cell receptor and IL-2signaling (Mikkers, et al., Mol. Cell. Biol., 2004, 6104). Although theexact mechanism of action of PIM kinases in an immunological setting hasyet to be fully defined, they have been reported to phosphorylate anumber of substrates involved in cellular proliferation,differentiation, and survival (Bullock et al., J. Biol. Chem., 2005280:41675; Chen et al., PNAS 2002 99:2175; Dautry et al. J. Biol. Chem.1998 263:17615).

Chronic and acute inflammatory and autoimmune diseases are associatedwith the overproduction of pro-inflammatory cytokines and activation ofimmune cells against the body's own tissues. However, many of thesediseases are not adequately treated by current therapies and/or thesetherapies have significant side effects/risks.

A particular example of an autoimmune disease is multiple sclerosis(MS). MS is a progressive central nervous system (CNS) inflammatoryautoimmune disease wherein the immune system mounts responses againstCNS components. The resulting damage to axons and nerves leads toprogressive neurological impairment and significant disability. MSaffects over 2.5 million people worldwide (www.nationalmssociety.org);however many current therapies are only moderately effective and havequestionable risk factors

A need therefore remains for compounds and methods for treatingautoimmune and inflammatory diseases.

International patent application, publication number WO 2004/058769discloses, inter alia, certain 3-aryl and 3-N-arylamino-substituted[1,2,4]triazolo[4,3-b]pyridazines purported to inhibit several proteinkinases, including PIM-1.

It has now been found that certain [1,2,4]triazole[4,3-a]pyridinecompounds bearing a quinolinyl group at the 3 position of thetriazolopyridine ring are inhibitors of PIM kinases, in particular PIM-1and/or PIM-2 and/or PIM-3 kinases, which are useful for treatingdiseases such as cancers and inflammatory diseases. In addition,compounds of the invention may be useful for treating immunecell-associated diseases and disorders, such as inflammatory andautoimmune diseases.

Accordingly, provided is a compound of the general Formula I:

or a pharmaceutically acceptable salt thereof, wherein:

R¹⁰ and R¹¹ together with the N to which they are attached form a 4-8membered heterocyclic ring optionally having an additional ringheteroatom selected from N and O, wherein the heterocyclic ring isoptionally substituted with one or more R⁹ groups;

each R⁹ is independently selected from halogen, (1-6C)alkyl,NR^(f)R^(g), -(1-6C alkyl)NR^(h)R^(i), OR^(j), (1-6C alkyl)OR^(k),C(O)NR^(m)R^(n), C(O)O(1-6C alkyl), and -(1-6C alkyl)NR^(h)C(O)O(1-6Calkyl);

B is H, OR^(a), (1-6C alkyl)NR^(b)R^(c), (1-6C alkyl)OH, NR^(b)R^(c), orCH(OH)CH₂OH;

R¹ is H, F, Cl, Br, methyl, ethyl, cyclopropyl or CN;

R^(1a), R², R³ and R⁴ are independently H, F, Cl, Br, methyl, ethyl,cyclopropyl or CN;

R⁵ and R⁷ are independently H, F, Me or CN;

R⁶ is H, F, Me, Br, CN, ethyl, cyclopropyl or phenyl;

R^(a) is H, (1-6C alkyl), -(1-6C alkyl)-O-(1-6C alkyl) or -(1-6Calkyl)-O-(3-6C cyclo alkyl);

each R^(b), R^(c), R^(d), R^(e), R^(f), R^(g), R^(h), R^(i), R^(j),R^(k), and R^(m) is independently selected from H and (1-6C alkyl); and

R^(n) is H, (1-6C alkyl) or O-(1-6C alkyl).

The term “C₁-C₆ alkyl” as used herein refers to saturated linear orbranched-chain monovalent hydrocarbon radicals of one to six carbonatoms, respectively. Examples include, but are not limited to, methyl,ethyl, 1-propyl, 2-propyl, 1-butyl, 2-methyl-1-propyl, 2-butyl,2-methyl-2-propyl, 2,2-dimethylpropyl, 1-pentyl, 2-pentyl, 3-pentyl,2-methyl-2-butyl, 3-methyl-2-butyl, 3-methyl-1-butyl, 2-methyl-1-butyl,1-hexyl, 2-hexyl, 3-hexyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl,4-methyl-2-pentyl, 3-methyl-3-pentyl, 2-methyl-3-pentyl,2,3-dimethyl-2-butyl, and 3,3-dimethyl-2-butyl.

In certain embodiments, R¹ is H.

In certain embodiments, R^(1a) is selected from H, F and Cl.

In certain embodiments, R^(1a) is H. In certain embodiments, R^(1a) isF. In certain embodiments, R^(1a) is Cl.

In certain embodiments, R² is selected from H and F.

In certain embodiments, R² is H. In other embodiments, R² is F.

In certain embodiments, R³ is H.

In certain embodiments, R⁴ is H.

In certain embodiments, R⁵ is H.

In certain embodiments, R⁶ is H.

In certain embodiments, R⁷ is H.

In certain embodiments, each of R⁵, R⁶ and R⁷ is H.

In certain embodiments, each of R¹, R^(1a), R², R³, R⁴, R⁵, R⁶ and R⁷ isH.

The phrase “R¹⁰ and R¹¹ together with the N to which they are attachedform a 4-8 membered heterocyclic ring” refers to a group having theformula —NR¹⁰R¹¹ representing a 4-8 membered saturated heterocyclicradical having at least one ring nitrogen atom. The heterocyclic ringoptionally has an additional ring heteroatom selected from N and O, theremaining ring atoms being carbon.

In certain embodiments, the —NR¹⁰R¹¹ group forms a 5-7 memberedheterocyclic ring. In certain embodiments, the heterocyclic ring issubstituted with one or more R⁹ groups, for example 1-4 R⁹ groups, andas a further example 1-2 R⁹ groups.

Particular examples of heterocyclic rings represented by —NR¹⁰R¹¹include piperidinyl, piperazinyl, and morpholinyl ring systems. Incertain embodiments, the heterocyclic ring is substituted by 1-4 R⁹groups, for example 1-2 R⁹ groups.

In certain embodiments, R⁹ is CF₃.

In certain embodiments, R⁹ is (1-6C)alkyl. Examples include methyl,ethyl, and propyl. A particular example is Me.

In certain embodiments, R⁹ is NR^(f)R^(g). Examples include groups whereR^(f) is H or Me and R^(g) is H, methyl, ethyl, propyl, isopropyl,butyl, or isobutyl. Particular values of R⁹ when represented byNR^(f)R^(g) include NH₂.

In certain embodiments, R⁹ is (1-6C alkyl)NR^(h)R^(i). Examples includegroups where R^(h) is H and R^(i) is H or alkyl. Particular values of R⁹when represented by -(1-6C alkyl)NR^(h)R^(i) include CH₂NR^(h)R^(i), forexample CH₂NH₂.

In certain embodiments, R⁹ is OR^(j). Examples include groups where is Hor (1-6C) alkyl, for example methyl. A particular example is OH.

In certain embodiments, R⁹ is (1-6C alkyl)OR^(k). Examples includegroups where R^(k) is H. A particular example is CH₂OH.

In certain embodiments, R⁹ is C(O)NR^(m)R^(n). Examples include groupswhere R^(m) and R^(n) are independently H or (1-6C) alkyl, for examplemethyl. A particular example is C(O)NH₂.

In certain embodiments, R⁹ is C(O)O(1-6C alkyl). Examples include CO₂Meand CO₂Et.

In certain embodiments, R⁹ is (1-6C alkyl)NR^(h)C(O)O(1-6C alkyl).Examples include groups where R^(h) is H. Particular values includeCH₂NR^(h)C(O)O(1-6C alkyl), for example CH₂NHCO₂t-Bu.

In certain embodiments, the heterocyclic ring represented by —NR¹⁰R¹¹ isa 5-7 membered heterocyclic ring which is unsubstituted or substitutedwith one or more R⁹ groups independently selected from Me, NH₂, CH₂NH₂,OH, CH₂OH, C(O)OMe, C(O)NH₂ and CH₂NHCO₂t-Bu. In certain embodiments,the heterocyclic ring represented by NR¹⁰R¹¹ is substituted with one ortwo of said R⁹ groups.

Particular embodiments of heterocyclic rings represented by —NR¹⁰R¹¹include the structures:

In certain embodiments, B is H.

In certain embodiments, B is OR^(a).

Examples of B when represented by OR^(a) include groups wherein R^(a) is(1-6C)alkyl. Particular values include OMe, OEt and O-(isobutyl).Particular mention is made of OMe.

Examples of B when represented by OR^(a) include groups wherein R^(a) is-(1-6C alkyl)-O-(1-6C alkyl). Particular values of OR^(a) include—OCH₂CH₂OMe and —OCH₂CH₂CH₂OMe. Particular mention is made of—OCH₂CH₂OMe.

Examples of B when represented by OR^(a) include groups wherein R^(a) is-(1-6C alkyl)-O-(3-6C cycloalkyl). A particular of OR^(a) includes—OCH₂CH₂O(cyclopropyl).

In certain embodiments, B is (1-6C alkyl)NR^(b)R^(c). In certainembodiments, R^(b) is H. In certain embodiments, R^(c) is H. In otherembodiments, R^(c) is (1-6C)alkyl. Examples include groups having theformula CH₂NR^(b)R^(c), for example CH₂NHEt and CH₂NH₂.

In certain embodiments, B is (1-6C alkyl)OH. A particular value of B isCH₂OH.

In certain embodiments, B is CH(OH)CH₂OH.

It will be appreciated that certain compounds according to the inventionmay contain one or more centers of asymmetry and may therefore beprepared and isolated in a mixture of isomers such as a racemic mixture,or in an enantiomerically pure form.

It will further be appreciated that the compounds of Formula I or theirsalts may be isolated in the form of solvates, and accordingly that anysuch solvate is included within the scope of the present invention.

The compounds of Formula I include pharmaceutically acceptable saltsthereof. In addition, the compounds of Formula I also include othersalts of such compounds which are not necessarily pharmaceuticallyacceptable salts, and which may be useful as intermediates for preparingand/or purifying compounds of Formula I and/or for separatingenantiomers of compounds of Formula I.

According to another aspect, the present invention provides a processfor the preparation of a compound of Formula I or a salt thereof asdefined herein which comprises:

(a) coupling a corresponding compound having the formula II

wherein L¹ represents a leaving atom or group, with a compound havingthe formula HNR¹⁰R¹¹ wherein NR¹⁰R¹¹ represents a 4-8 memberedheterocyclic ring optionally having an additional heteroatom selectedfrom N and O and optionally substituted with one or more R⁹ groups,using a palladium catalyst and a ligand in the presence of a base; or

(b) reacting a compound of Formula III

with a compound having the Formula IV

in the presence of an organo hypervalent iodine reagent; or

(c) for a compound of Formula I where B is —OR^(a), reacting acorresponding compound having the Formula V

with a compound of the formula R^(a)-L², wherein L² represents a leavingatom or group, in the presence of a base;

(d) for a compound of Formula I wherein B is —(CH₂)NR^(b)R^(c), reactinga corresponding compound having the Formula VI

with hydrazine; and

removing any protecting group or groups and, if desired, forming a salt.

Referring to method (a), the leaving atom L¹ may be, for example, ahalide such as Br or I. Alternatively, L¹ may be a leaving group, suchas a hydrocarbylsulfonyloxy group, for example, a triflate group, or anarylsulfonyloxy group or an alkylsulfonyloxy group, such as a tosylateor a mesylate group. Suitable palladium catalysts include Pd₂(dba)₃ andPd(OAc)₂. Suitable ligands include rac-BINAP or DIPHOS. The base may be,for example, an alkali metal carbonate or alkoxide, such as for examplecesium carbonate or sodium tert-butoxide. Convenient solvents includeaprotic solvents such as ethers (for example tetrahydrofuran orp-dioxane) or toluene. The coupling of a compound of formula (II) withHNR¹⁰R¹¹ can be conveniently performed at a temperature between 0° C.and reflux, and more particularly at reflux.

Referring to method (b), the organo hypervalent iodine reagent refers toany hypervalent iodine reagent suitable for forming heterocyclic rings.Examples include iodobenzene diacetate (IBD) and[hydroxy(tosyloxy)iodo]benzene (HTIB), which can be prepared by treatingIBD with p-toluenesulfonic acid monohydrate in acetonitrile. Suitablesolvent systems when using IBD include methanolic potassium hydroxide.Suitable solvent systems when using HTIB include neutral solvents, forexample acetonitrile or dioxane. The reaction can be performed at atemperature ranging from 0 to 60° C.

Referring to method (c), the leaving atom L² may be, for example ahalogen atom such as Br, Cl or I. Alternatively, L² can be a leavinggroup, for example an arylsulfonyloxy group or an alkylsulfonyloxygroup, such as a mesylate or a tosylate group. The base may be, forexample, an alkali metal hydride or carbonate, such as sodium hydride,potassium hydride, sodium carbonate, potassium carbonate or cesiumcarbonate. Convenient solvents include aprotic solvents such as ethers(for example tetrahydrofuran or p-dioxane), DMF, or acetone. Thereaction can be conveniently performed at a temperature ranging from −78to 100° C.

Referring to method (d), the reaction is conveniently performed atambient temperature. Suitable solvents include alcohols such asmethanol.

A compound of Formula II

can be prepared by cyclizing a corresponding compound having the formulaVII

where P¹ is an alcohol protecting group, in the presence of an organohypervalent iodine reagent as described above, followed by deprotectionof the alcohol group and conversion of the alcohol group to analkylsulfonyloxy group, such as a triflate group.

The compounds of the formulas II and VII are believed to be novel andare provided as further aspects of the invention.

The ability of test compounds to act as PIM-1, PIM-2 or PIM-3 inhibitorsmay be demonstrated by the assay described in Examples A, B and C,respectively.

Compounds of Formula I have been found to be inhibitors of PIM-1 and/orPIM-2 and/or PIM-3, and are useful for treating diseases and disorderswhich can be treated with a PIM-1 and/or PIM-2 and/or PIM-3 kinaseinhibitor, including diseases mediated by PIM-1 and/or PIM-2 and/orPIM-3 kinases. Particular compounds of this invention are inhibitors ofPIM-1 and therefore are useful in treating diseases and disordersmediated by PIM-1, such as cancers, such as hematological cancers andsolid tumors (e.g., breast cancer, colon cancer, gliomas).

Examples of hematological cancers include, for instance, leukemias,lymphomas (non-Hodgkin's lymphoma), Hodgkin's disease (also calledHodgkin's lymphoma), and myeloma, for instance, acute lymphocyticleukemia (ALL), acute myeloid leukemia (AML), acute promyelocyticleukemia (APL), chronic lymphocytic leukemia (CLL), chronic myeloidleukemia (CML), chronic neutrophilic leukemia (CNL), acuteundifferentiated leukemia (AUL), anaplastic large-cell lymphoma (ALCL),prolymphocytic leukemia (PML), juvenile myelomonocyctic leukemia (JMML),adult T-cell ALL, AML with trilineage myelodysplasia (AML/TMDS), mixedlineage leukemia (MLL), myelodysplastic syndromes (MDSs),myeloproliferative disorders (MPD), and multiple myeloma (MM).Additional examples of hematological cancers include myeloproliferativedisorders (MPD) such as polycythemia vera (PV), essentialthrombocytopenia (ET) and idiopathic primary myelofibrosis(IMF/IPF/PMF). Certain cancers which can be treated with compounds ofFormula I are cancers which of hematological origin, such as, but notlimited to, cancers derived from T cells or B cells.

In addition, certain compounds according to the present invention may beuseful for the treatment of inflammatory disorders mediated by T and Bcells function, such as rheumatoid arthritis, lupus, multiple sclerosis,and inflammatory bowel disease.

Accordingly, a further embodiment of this invention provides a method oftreating cancer in a mammal in need thereof, comprising administering tosaid mammal a compound of Formula I or a pharmaceutically acceptablesalt thereof. In one embodiment, the cancer is of hematological origin.In one embodiment, the cancer derives from T cells. In one embodiment,the cancer derives from B cells.

A further embodiment of this invention provides a compound of Formula Ior a pharmaceutically acceptable salt thereof, for use in the treatmentof cancer. In one embodiment, the cancer is of hematological origin. Inone embodiment, the cancer derives from T cells. In one embodiment, thecancer derives from B cells.

Another embodiment of this invention provides a method of treating orpreventing inflammatory and autoimmune diseases, comprisingadministering to a mammal in need thereof an effective amount of acompound of Formula I. Examples of diseases which can be treated includeinflammatory or autoimmune diseases. In one embodiment, the disease ismultiple sclerosis. In another embodiment, the disease is lupus. Inanother embodiment, the disease is inflammatory bowel disease.

A further embodiment of this invention provides a compound of Formula Ifor use in treating an inflammatory or autoimmune disease. In oneembodiment, the disease is multiple sclerosis. In another embodiment,the disease is lupus. In another embodiment, the disease is inflammatorybowel disease.

Expression of PIM kinases in immune cells can be induced by cytokinespresent during immune responses. Immune cells are critically dependenton cytokines for differentiation and development of effector functionsduring normal and pathogenic immune responses. Thus, compounds of theinvention may be useful for treating diseases and disorderscharacterized by aberrant cytokine production and responses and/oraberrant immune cell activation.

Accordingly, another embodiment of the invention provides a method oftreating diseases and disorders characterized by aberrant cytokineproduction and responses and/or aberrant immune cell activation in amammal in need thereof, comprising administering to the mammal acompound of Formula I or a pharmaceutically acceptable salt thereof.

Examples of disease and disorders which can be treated using a compoundof Formula I include transplant rejection and autoimmune andinflammatory diseases and disorders. Examples of autoimmune diseases anddisorders include multiple sclerosis (MS), systemic lupus erythematosis,inflammatory bowel disease (IBD), Crohn's disease, irritable bowelsyndrome, pancreatitis, ulcerative colitis, diverticulosis, Grave'sdisease, arthritis (including rheumatoid arthritis, juvenile rheumatoidarthritis, osteoarthritis, psoriatic arthritis and ankylosingspondylitis), myasthenia gravis, vasculitis, autoimmune thyroiditis,dermatitis (including atopic dermatitis and eczematous dermatitis),psoriasis, scleroderma, asthma, allergy, systemic sclerosis, vitiligo,graft vs. host disease (GVHD), Sjogren's syndrome, glomerulonephritis,IgA nephoropathy, diabetes mellitus (type I) and asthma.

Particular examples of diseases and disorders which can be treated usinga compound of Formula I include autoimmune and inflammatory diseases.Particular examples of such diseases include asthma, MS, inflammatorybowel disease (IBD), lupus, psoriasis and rheumatoid arthritis.

Another embodiment provides a compound of Formula I or apharmaceutically acceptable salt thereof for use in the treatment ofdiseases and disorders characterized by aberrant cytokine production andresponses and/or aberrant immune cell activation in a mammal. Examplesof such diseases and disorders include autoimmune and inflammatorydiseases.

A subset of the triazolopyridine compounds disclosed herein was found tohave an IC₅₀ for PIM-1 that is at least 10 fold less than the IC₅₀ forPIM-2. As a further example, particular triazolopyridine compoundsdisclosed herein were found to have an IC₅₀ for PIM—that is at least 100fold less than the IC₅₀ for PIM-2. Accordingly, also provided aretriazolopyridine compounds which are highly potent PIM-1 inhibitors andare highly selective for PIM-1 relative to PIM-2.

A subset of the triazolopyridine compounds disclosed herein were foundto have an IC₅₀ for PIM-1 that is at least 10 fold less than the IC₅₀for PIM-2 and an IC₅₀ for PIM-3 approximately equivalent to thatobserved for PIM-1. As a further example, particular triazolopyridinecompounds disclosed herein were found to have an IC₅₀ for PIM-1 that isat least 100 fold less than the IC₅₀ for PIM-2, and IC₅₀ for PIM-3approximately equivalent to that observed for PIM-1. Accordingly, alsoprovided are triazolopyridine compounds which are highly potentPIM-1/PIM-3 dual inhibitors and are highly selective for PIM-1 and PIM-3relative to PIM-2.

As used herein, the term treatment includes prophylaxis as well astreatment of an existing condition.

Accordingly, another aspect of this invention provides a method oftreating diseases or medical conditions in a mammal mediated by a PIM-1and/or PIM-2 and/or PIM-3 kinase, comprising administering to saidmammal one or more compounds of Formula I or a pharmaceuticallyacceptable salt or prodrug thereof in an amount effective to treat orprevent said disorder.

Compounds of Formula I may also be useful as adjuvants to cancertreatment, that is, they can be used in combination with one or moreadditional drugs, for example a chemotherapeutic that works by the sameor by a different mechanism of action.

Compounds of the present invention may also be used in combination withone or more additional drugs, for example an anti-inflammatory compound,an immunosuppressive compound or an immunodepleting agent that works bythe same or a different mechanism of action.

The phrase “effective amount” means an amount of compound that, whenadministered to a mammal in need of such treatment, is sufficient to (i)treat or prevent a particular disease, condition, or disorder mediatedby a PIM-1 and/or PIM-2 and/or PIM-3 kinase, (ii) attenuate, ameliorate,or eliminate one or more symptoms of the particular disease, condition,or disorder, or (iii) prevent or delay the onset of one or more symptomsof the particular disease, condition, or disorder described herein.

The amount of a compound of Formula I that will correspond to such anamount will vary depending upon factors such as the particular compound,disease condition and its severity, the identity (e.g., weight) of themammal in need of treatment, but can nevertheless be routinelydetermined by one skilled in the art.

As used herein, the term “mammal” refers to a warm-blooded animal thathas or is at risk of developing a disease described herein and includes,but is not limited to, guinea pigs, dogs, cats, rats, mice, hamsters,and primates, including humans.

Compounds of the invention may be administered by any convenient route,e.g. into the gastrointestinal tract (e.g. rectally or orally), thenose, lungs, musculature or vasculature, or transdermally or dermally.Compounds may be administered in any convenient administrative form,e.g. tablets, powders, capsules, solutions, dispersions, suspensions,syrups, sprays, suppositories, gels, emulsions, patches etc. Suchcompositions may contain components conventional in pharmaceuticalpreparations, e.g. diluents, carriers, pH modifiers, sweeteners, bulkingagents, and further active agents. If parenteral administration isdesired, the compositions will be sterile and in a solution orsuspension form suitable for injection or infusion. Such compositionsform a further aspect of the invention.

According to another aspect, the present invention provides apharmaceutical composition, which comprises a compound of Formula I or apharmaceutically acceptable salt thereof, as defined hereinabove. In oneembodiment, the pharmaceutical composition includes the compound ofFormula I together with a pharmaceutically acceptable diluent orcarrier.

According to another aspect, the present invention provides a compoundof Formula I or a pharmaceutically acceptable salt thereof, for use intherapy, such as the treatment of a PIM-1 and/or PIM-2 and/or PIM-3kinase-mediated condition.

According to a further aspect, the present invention provides the use ofa compound of Formula I, or a pharmaceutically acceptable salt thereof,for use in the treatment of a PIM-1 and/or PIM-2 and/or PIM-3kinase-mediated condition, as defined hereinabove.

EXAMPLES

The following examples illustrate the invention. In the examplesdescribed below, unless otherwise indicated all temperatures are setforth in degrees Celsius. Reagents were purchased from commercialsuppliers such as Aldrich Chemical Company, Lancaster, TCI or Maybridge,and were used without further purification unless otherwise indicated.Tetrahydrofuran (THF), dichloromethane (DCM, methylene chloride),toluene, and dioxane were purchased from Aldrich in Sure seal bottlesand used as received.

The reactions set forth below were done generally under a positivepressure of nitrogen or argon or with a drying tube (unless otherwisestated) in anhydrous solvents, and the reaction flasks were typicallyfitted with rubber septa for the introduction of substrates and reagentsvia syringe. Glassware was oven dried and/or heat dried.

Column chromatography was done on a Biotage system (Manufacturer: DyaxCorporation) having a silica gel column or on a silica SepPak cartridge(Waters).

Example A Enzyme PIM-1 Assay

The assay for the determination of PIM-1 activity is based on theincorporation of [³³P]PO₄ from [γ-³³P]ATP into PIM2tide substrate andcapture of the radiolabeled peptide onto a Whatman P81(phosphocellulose) filter plate. The amount of radiolabeled product isthen measured by liquid scintillation counting. The final bufferconditions were as follows: 20 mM K⁺MOPS, pH 7.4, 10 mM MgCl₂, 0.005%Tween-20, 1 mM DTT. Assay mixtures contained 35 μM [γ-³³P]ATP (20pfi/mL), 7.5 μM PIM2tide and 0.25 nM PIM-1 in a total volume of 50 μL.Incubations were carried out for 60 min at 22° C. and quenched with 75μL of 200 mM H₃PO₄, filtered through a Whatman P81 plate and washed(1×200 μL and 5×100 μL) with 200 mM H₃PO₄. Fifty μL of liquidscintillation cocktail were then added per well, and the plate wascounted for 30 s/well using a TopCount NXT.

IC₅₀ Determinations:

Compounds were prepared at 50× the final concentration in DMSO byconducting 3-fold serial dilutions from a 500-μM intermediate dilutionto give a 10-point dosing curve having a high dose of 10 μM. One-μLaliquots of these were then transferred to the assay mixtures above togive a final concentration of DMSO of 2%. A standard or referencecompound was typically included on each assay plate to validate thatplate. For each plate, percent of control (POC) values were calculatedfor each well. IC₅₀'s were estimated from the POC's using a standard4-parameter logistic model. The IC₅₀ is defined as the concentration ofinhibitor at which the POC equals 50 for the fitted curve. Compounds ofFormula I were found to have an average IC₅₀ below 10 μM when tested inthis assay. Specific IC₅₀ values are provided in Table 1.

Example B PIM-2 Assay

Assay was performed as described in Example A, using 4 μM [γ-³³P]ATP (20μCi/mL), 1.0 μM PIM2tide and 1.5 nM GST-tagged recombinant full-lengthhuman Pim-2 in place of PIM-1. Compounds of Formula I were found to havean average IC₅₀ below 10 μM when tested in this assay. Specific IC₅₀values are provided in Table 1.

Example C PIM-3 Assay

Assay was performed as described in Example A, using 30 μM [γ-³³P]ATP(20 μCi/mL), 3.75 μM PIM2tide and 0.5 nM recombinant rat PIM-3 in placeof PIM-1. Compounds of Formula I were found to have an average IC₅₀below 10 μM when tested in this assay. Specific IC₅₀ values are providedin Table 1.

TABLE 1 Example PIM-1 PIM-2 PIM-3 No. IC₅₀ (nM) IC₅₀ (nM) IC₅₀ (nM) 1255 10000 554 2 179 10000 ND 3 24 2305 60 4 2793 10000 ND 5 24 2598 1216 31 2977 105 7 17 3591 105 8 54 10000 189 9 11 543 24 10 3 224 13 11 842600 217 12 3 92 4 13 2 29 4 14 4 509 20 15 15 2080 ND 16 3 185 10 17 771958 51 18 32 736 25 19 18 1798 ND 20 48 2379 ND 21 7 142 ND 22 270 3097ND 23 22 647 62 24 150 10000 ND 25 128 3022 ND 26 21 613 62 27 34 570 38ND: Not determined

Example D Cellular Proliferation Assay

The assay for determination of the antiproliferative activity ofmultiple PIM inhibitors in the JAK2-driven cell lines is conducted asfollows. Cells are plated out to 96-well plates at an initial density of10,000 cells/well in 95 μL. Compounds are prepared at 20× the finalconcentration in DMSO by conducting 3-fold serial dilutions to give a10-point dosing curve having a high dose of 1000 μM. Aliquots (5 μL) ofthese dilutions are then transferred to the appropriate wells of the96-well plates containing cells to yield a final DMSO concentration of0.5%. The cells are then incubated with compound for 72 hours at 37° C.,5% CO₂. CelltiterBlue reagent (Promega, Catalog #: G8080) is then added(20 μL/well) and incubated at 37° C., 5% CO₂ for 1-8 hours depending onthe cell line being analyzed. The plate is then quantified employing afluorescence plate reader (Model: Gemini [Molecular Devices]; Settings:560 nm (Ex)/590 nm (Em) 570 nm (cut-off) [CellTiter Blue Assay].

The values for each well are then converted to a percent of untreatedcontrol (POC). These POC values are then plotted as a function ofcompound concentration. A 4-parameter curve-fit analysis is performedfor each compound dilution and an IC₅₀ value is calculated from thiscurve.

Example E T Cell In Vitro Functional Assays

The in vitro assays which can be used to assess the effects of thecompounds of the invention are described in assays A, B, C and D below.CD4+ T cells are isolated from red blood cell-depleted splenocytes ofC57B1/6J mice (Jackson Laboratories, catalog #000664) using CD4+ T cellisolation kit (Miltenyi, catalog #130-090-860).

In assay (A), purified CD4+ T cells are plated in 96 well plates at90000 cells/well in 90 μL. A dilution series of the compounds areprepared at 100× the final concentration in DMSO and then diluted10-fold into complete media (10× stocks). 10 μL of 10× compound stocksare added to appropriate wells of 96 well plates containing cells andincubated for 1 hour at 37° C., 5% CO₂. The cell/compound mixtures arethen transferred to a 96 well plate coated with anti-CD3 mAb (1 μg/mL;BD Pharmingen, catalog #553057) and soluble anti-CD28 mAb (1 μg/mL; BDPharmingen, catalog #553294) was added. Plates are cultured at 37° C.,5% CO₂ for 40 hours. 20 μL of the culture are removed for determinationof proliferation using the CellTitre-Glo™ luminescent assay (Promega,Catalog #G7571) according to the manufacturer's protocol. The plate isquantified on a Packard TopCount instrument using luminescence protocoland data analyzed using Prism software.

In assay (B), purified CD4+ cells are treated with compound andstimulated as described for assay (A). After 40 hours, supernatants areassayed for IL-2 using R&D duo set ELISA kits (catalog #DY402). ELISAplates are quantified relative to a standard curve using MolecularDevices Versamax Reader at 450 nM and Softmax Pro software.

In assay (C), 1,000,000 cells/mL of purified CD4+ T cells are mixed with1 μg/mL anti-CD28, 10 ng/mL IL-4 (R&D Systems cat #404-ML-010/CF) and 2μg/mL anti-IFNγ (R&D Systems catalog #AB-485-NA) and placed into platescoated with 1 μg/mL anti-CD3. After 5 days, cells are harvested, washedand incubated overnight at 37° C., 5% CO₂. The following day, 50,000cells are plated into each well of a 96 well plate. A dilution series ofcompounds are prepared at 200× the final concentration in DMSO, then 10×stocks are prepared by dilution in cell culture media. 10 μL of 10×stocks are added to the cells in the 96-well plate and incubated for 2hours at 37° C., 5% CO₂. Cell/compound mixtures are then transferred towell coated with 0.1 μg anti-CD3 and incubated at 37° C., 5% CO₂.Culture supernatants are removed 18 hours later and tested for IL-4levels by ELISA (R&D Systems catalog #DY404). ELISA plates arequantified relative to a standard curve using Molecular Devices VersamaxReader at 450 nM and Softmax Pro software.

In assay (D), 1,000,000 cells/mL of purified CD4+ T cells are mixed with1 μg/mL anti-CD28, 50 ng/mL IL-6 (R&D Systems cat #406-ML-025/CF), 1ng/mL TGFβ (R&D Systems cat #303-B2-002), 2 μg/mL anti-IL-4 (R&D Systemscatalog #AB-404-NA), 2 μg/mL anti-IFNγ (R&D Systems catalog #AB-485-NA)and placed into plates coated with 1 μg/mL anti-CD3. After 4 days, cellsare harvested, washed and 100,000 cells are plated into 96 well plate. Adilution series of compounds are prepared at 200× the finalconcentration in DMSO, then 10× stocks are prepared by dilution in cellculture media. 10 μL of 10× stocks are added to the cells in the 96 wellplate. After 2 hours, 50 ng IL-23 (R&D Systems catalog #1887-ML-010/CF)is added to each well and 18 hours later supernatants are removed andtested for IL-22 levels by ELISA (R&D Systems catalog #M2200). ELISAplates are quantified relative to a standard curve using MolecularDevices Versamax Reader at 450 nM and Softmax Pro software.

Example F T Cell In Vivo Functional Assay

The effect of compounds of Formula I on T cell responses can bedetermined by the following experiment. On Day 0, C57BL/6 (JacksonLaboratories #000664, 6-8 weeks of age) are immunized at the base of thetail with 100 μg of hen egg lysozyme (HEL; Sigma #L7773) with completeFreund's adjuvant (CFA; Sigma #F5881). Starting on Day 0 and continuinguntil Day 7, mice are dosed twice a day by oral administration withvehicle (water) or the compound of Formula I (200 mg/kg). On Day 7,popiteal lymph nodes are removed, single cell suspensions are preparedand 500,000 cells in 200 μL are activated in 96 well plates with theindicated dose of HEL peptide. Following incubation for 72 hours at 37°C., 5% CO₂, supernatants are harvested for IFNγ ELISA (R&D Systemscatalog #MIF00) and proliferation is assessed using the CellTitre-Glo™luminescent assay (Promega, Catalog #G7571) with both assays performedaccording to the manufacturer's protocol. ELISA plates are quantifiedrelative to a standard curve using Molecular Devices Versamax Reader at450 nM and Softmax Pro software; proliferation was quantitated on aPackard TopCount instrument using luminescence protocol and dataanalyzed using excel software.

Example G B Cell In Vivo Functional Assay

The effect of a compound of Formula I on B cell responses can bedetermined with the following experiment. On Day 0, C57BL/6J mice(Jackson Laboratories #000664, 6-8 weeks of age) are immunized at thebase of the tail with 20 μg of hen egg lysozyme (HEL; Sigma #L7773) withcomplete Freund's adjuvant (CFA; Sigma #F5881). Mice are re-immunized onday 7 with 20 μg HEL in alum (Pierce catalog #77161). Starting on Day 0and continuing through Day 28, mice are dosed once a day by oraladministration with vehicle (water) or the compound of Formula I (200mg/kg). Serum is collected on days 0, 7, 14, 21, and 28 and analyzed forHEL-specific total IgG, IgG1, IgG2a, IgG2b, and IgG3 antibody productionby capture ELISA (antibodies purchased from Invitrogen, catalog Nos.M30007, M32107, M32307, M32507 and M32607). ELISA plates are quantitatedusing Molecular Devices Versamax reader at 450 nM. The group mean titerof each antibody analyte is converted to percent of vehicle control(=100%).

Example H Adoptive Transfer Experimental Autoimmune Encephalomyelitis

The effect of a compound of Formula I on an autoimmune disease inducedby T cells can be determined using an adoptive transfer EAE model, ananimal model of human multiple sclerosis (Brain (2006), 129, 1953-1971).This model relies on the injection of T cells from animals with EAE intodisease-free host animals. This injection of cells is known to thoseskilled in the art as adoptive transfer. By injecting the animals withactivated, encephalogenic T cells, this model is focused on thepathogenic stage of EAE autoimmune disease. On Day −14, C57BL/6 mice(Taconic Farms; 10 weeks old) are immunized with a disease-causingprotein, MOG(35-55) peptide in complete Freund's adjuvant (HookeLaboratories, catalog #EK-0113). On Day −3, spleens are harvested,single cell suspensions are prepared and then 5,000,000 cells/mL arestimulated with 20 μg/mL MOG(33-55) peptide (Open Biosystems), 30 ng/mLIL-12 (R&D Systems catalog #419-ML-010), 10 μg/mL anti-IFNγ antibody (BDBiosciences catalog #554408) at 37° C., 5% CO₂. On Day 0, 1,500,000 ofthese cells are injected intravenously into the tail veins of C57BL/6recipient mice. The recipient mice are divided into treatment groups forvehicle (distilled water; 10 mL/kg) or the compound of Formula I (200mg/kg), both administered by oral gavage twice daily for 26 days. Therecipient mice are scored daily days 0 through 26 using the followingclinical scoring system:

0.0—no symptoms

1.0—limp tail

2.0—limp tail and weakness of hind legs

3.0—limp tail and complete hind limb paralysis, or partial front andhind limb paralysis, or severe head tilting combined with pushingagainst cage wall and spinning when picked up by tail

4.0—limp tail, complete hind limb paralysis and partial front limbparalysis

5.0—Full body paralysis, or spontaneous rolling or found dead due toparalysis

Example I MOG(35-55)-Induced Experimental Autoimmune Encephalomyelitis

An additional method of determining the effect of compounds of Formula Ion an autoimmune disease associated with T cells and cytokines uses theMOG-induced experimental autoimmune encephalomyelitis (EAE) model.MOG-induced EAE is an animal model of human multiple sclerosis (Brain(2006), 129, 1953-1971).

On Day 0, C57BL/6J mice (Jackson Laboratories #000664, 6-8 weeks of age)are injected subcutaneously with 100 μL of complete Freund's adjuvant(CFA) prepared as a 1:1 emulsion of (a) incomplete Freund's adjuvant(Difco, catalog #263910) containing 8 mg/mL M. tuberculosis H37RA(Difco, catalog #231141) and (b) phosphate buffered saline (PBS)containing 1 mg/mL MOG(35-55) peptide (California Peptide Research Inc).On the day 0 and 2, mice are injected intravenously with 200 ng ofpertussis toxin (List Biological Laboratories, catalog #181). On day 7,the mice are randomized into treatment groups which received vehicle(distilled water) or the compound of Formula I (200 mg/kg) administeredby oral gavage twice daily from days 7 through 27.

The mice are scored daily on days 7 through 37 using the followingclinical scoring system:

0.0—no symptoms

0.5—tail weakness

1.0—limp tail

1.5—unsteady gait, mild hind limb ataxia

2.0—partial hind limb paralysis (hind limbs carrying weight)

2.5—partial hind limb paralysis (hind limbs not carrying weight)

3.0—full hind limb paralysis

3.5—full hind limb paralysis and partial front limb paralysis

4.0—full body paralysis

Example J CD4+CD45RBhi Adoptive Transfer Inflammatory Bowel Disease

The following adoptive transfer model of inflammatory bowel disease(IBD) can be performed to determine the effect of compounds of Formula Ion IBD, which is an autoimmune disease associated with T cells andcytokines

On Day 0, CD4+ T cells are isolated from the spleens of femaleBalb/cAnNCrl mice (Charles River Laboratories; 12 weeks old) asdescribed in Example E. The resulting cells are labeled with fluorescentantibodies against CD4 and CD45 markers and are sorted by flow cytometryfor CD4+CD45RBhi cells based on fluorescence. 400,000 CD4+CD45RBhi cellsare then injected intraperitoneally intoC.B17/Icr-Prkdc^(scid)/IcrIcoCrl mice (Charles River Laboratories straincode 236; 12 weeks old). This injection of cells is known to thoseskilled in the art as “adoptive transfer”. On Day 21, mice arerandomized into groups for oral gavage treatment with vehicle (1%carboxymethylcellulose sodium (CMC)/0.5% Tween 80 once daily; CMC, Sigmacatalog #C9481, Tween 80 Sigma catalog #P1754) or the compound ofFormula I (200 mg/kg; twice daily). Treatments continued through Day 42.

At the conclusion of the study, mice are sacrificed and the distal halfof their colons are placed in 10% neutral buffered formalin (RichardAllen Scientific catalog #53120-1) and paraffin embedded, sectioned into4 μm slices and stained with hematoxylin and eosin (H&E) for analysis bya board certified veterinary pathologist.

For each H&E stained section, submucosal edema is quantitated bymeasuring the distance from the muscularis mucosa to the internal borderof the outer muscle layer in a non-tangential area thought to mostrepresentative the severity of this change. Mucosal thickness is alsomeasured in a non-tangential area of the section that best representedthe overall mucosal thickness. This parameter is indicative of glandelongation and mucosal hyperplasia. The extent of inflammation(macrophage, lymphocyte and polymorphonuclear leukocyte (PMN)infiltrate) is assigned severity scores according to the followingcriteria:

Normal=0

Minimal=1 (generally focal affecting 1-10% of mucosa or if diffuse thenminimal)

Mild=2 (generally focal affecting 11-25% of mucosa or if diffuse thenmild)

Moderate=3 (26-50% of mucosa affected with areas of gland loss replacedby inflammatory cell infiltrate, milder in remaining areas of mucosa)

Marked=4 (51-75% of mucosa affected with areas of gland loss replaced byinflammatory cell infiltrate, milder in remaining areas of mucosa)

Severe=5 (76-100% of mucosa affected with areas of gland loss replacedby inflammatory cell infiltrate, milder in remaining areas of mucosa)

The parameters reflecting epithelial cell loss/damage are scoredindividually using a % area involved scoring method:

None=0

1-10% of the mucosa affected=1

11-25% of the mucosa affected=2

26-50% of the mucosa affected=3

51-75% of the mucosa affected=4

76-100% of the mucosa affected=5

Parameters that are scored using % involvement included: colon glandularepithelial loss (this includes crypt epithelial as well as remaininggland epithelial loss), and colon erosion (this reflects loss of surfaceepithelium and generally is associated with mucosal hemorrhage(reflective of the bleeding seen clinically and at necropsy).

The three scored parameters (inflammation, glandular epithelial loss,and erosion) are ultimately summed to arrive at a sum of histopathologyscores, which indicates the overall damage and would have a maximumscore of 15.

Example K MRL/lpr Lupus Model

MRL/lpr is considered to be an animal model of systemic lupuserythematosus (SLE), an autoimmune disease (Cohen and Maldonado 2003,Current Protocols in Immunology Chapter 15, Unit 15.20). MRL/lpr micehave a defect in the apoptosis of activated lymphocytes and over timedevelop a spontaneous and severe lymphoproliferative disordercharacterized by enlarged lymphoid organs, auto-antibody production andkidney disease resulting in proteinuria. SLE patients also exhibitauto-antibodies, and some patients develop kidney disease. To determinethe effect of compounds of Formula I in this model of SLE, the followingexperiment can be conducted.

MRL/MpJ-Fas<lpr> and age-matched MRL/MpJ control mice (JacksonLaboratories, catalog #000485 and #000486, respectively) are treatedonce daily with vehicle (1% CMC/0.5% Tween 80) or twice daily with thecompound of Formula I (200 mg/kg) for 10 weeks. Body weights,lymphadenopathy and urine protein levels are monitored weekly. Urineprotein levels are determined with Bayer Albustix dipsticks (Bayercatalog #2191) and scored according to the following scale:

0=none detected

0.5=trace amounts

1=30 mg/dL

2=100 mg/dL

3=300 mg/dL

4=2000 mg/dL

Serum levels of anti-ds-DNA antibody are measured by ELISA (AlphaDiagnostic, catalog #5120) on Day 28 and upon study termination. ELISAplates are quantitated using a Molecular Devices Versamax plate readerat 450 nM and titers calculated relative using to a standard curve usinga 4-parameter curve fit with Softmax Pro software.

Example 1

2-(7-(2-Methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)-8-(piperazin-1-yl)quinolineStep A: Preparation of 2-chloro-4-(2-methoxyethoxy)pyridine

Potassium 2-methylpropan-2-olate (4.214 g, 35.678 mmol) was slowly addedunder an atmosphere of dry N₂ to a solution of 2-chloro-4-nitropyridine(5.142 g, 32.434 mmol) in 2-methoxyethanol (40.0 mL, 506.74 mmol). Thereaction was stirred at ambient temperature for 2 hours and thenconcentrated under reduced pressure. The resulting oil was diluted withwater (200 mL) and extracted with EtOAc. The combined organic phaseswere dried over Na₂SO₄, filtered and concentrated under reduced pressureto give 5.36 g (88%) of desired product as a colorless oil.

Step B: Preparation of 2-hydrazinyl-4-(2-methoxyethoxy)pyridine

Hydrazine (10 mL, 318.6 mmol) was added to a solution of2-chloro-4-(2-methoxyethoxy)pyridine (1.00 g, 5.330 mmol) in pyridine(25 mL). The reaction was heated to reflux. After 18 hours the reactionmixture was partitioned between H₂O and DCM and the aqueous phase wasextracted with DCM. The combined organic extracts were dried overNa₂SO₄, filtered and concentrated under reduced pressure. The residuewas purified via flash column chromatography (40:1 DCM/MeOH followed by20:1 DCM/MeOH) to provide 320 mg (33%) of desired product as a whitesolid.

Step C: Preparation of8-(tert-butyldimethylsilyloxy)quinoline-2-carbaldehyde

8-Hydroxyquinoline-2-carbaldehyde (5.00 g, 28.9 mmol) and imidazole(4.32 g, 63.5 mmol) were dissolved in DCM (50 mL) under N₂. The reactionmixture was cooled to 0° C. after which timetert-butylchlorodimethylsilane (4.94 g, 31.8 mmol) was added. Afterstirring for 16 hours at ambient temperature, the reaction mixture waspartitioned between DCM and H₂O. The organic layer was washed with H₂Oand aqueous saturated NaHCO₃, dried over Na₂SO₄, filtered andconcentrated under reduced pressure to afford an orange oil. The residuewas purified via flash column chromatography (10:1 Hexane/EtOAc) toprovide 6.85 g (83%) of desired product as a yellow/orange oil.

Step D: Preparation of(E)-8-(tert-butyldimethylsilyloxy)-2-((2-(4-(2-methoxyethoxy)pyridin-2-yl)hydrazono)methyl)quinoline

2-Hydrazinyl-4-(2-methoxyethoxy)pyridine (0.076 g, 0.415 mmol) and8-(tert-butyldimethylsilyloxy) quinoline-2-carbaldehyde (0.119 g, 0.415mmol) were heated to reflux in EtOH (2 mL) for 16 hours. After coolingto ambient temperature the reaction mixture was filtered and washed withEtOH to provide 105 mg (56%) of desired product as an orange solid.

Step E: Preparation of8-(tert-butyldimethylsilyloxy)-2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinoline

To a solution of(E)-8-(tert-butyldimethylsilyloxy)-2-((2-(4-(2-methoxyethoxy)pyridin-2-yl)hydrazono)methyl)quinoline (0.101 g, 0.223 mmol) in DCM (1.0 mL) was added iodosobenzenediacetate (0.0719 g, 0.223 mmol). After stirring at ambient temperaturefor 5 hours the reaction mixture was partitioned between DCM and aqueoussaturated Na₂S₂O₃. The organic layer was washed with brine, dried overNa₂SO₄, filtered and concentrated under reduced pressure. The residuewas purified via flash column chromatography (40:1 DCM/MeOH) to provide70 mg (70%) of desired product.

Step F: Preparation of2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-ol

To a solution of8-(tert-butyldimethylsilyloxy)-2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinoline(0.070 g, 0.155 mmol) in THF (8 mL) was added 1M HCl (1.5 mL, 1.50mmol). After stirring at ambient temperature for 2 hours 1 M HCl (5 mL)was added and the reaction mixture stirred for a further 16 hours. Themixture was neutralized with 1M NaOH and diluted with EtOAc. The organiclayer was washed with brine, dried over Na₂SO₄, filtered andconcentrated under reduced pressure to give 40 mg (77%) of desiredproduct.

Step G: Preparation of2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yltrifluoromethanesulfonate

2-(7-(2-M ethoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-ol(0.040 g, 0.119 mmol), N-phenyltriflimide (0.0467 g, 0.131 mmol) and TEA(0.0365 mL, 0.262 mmol) were dissolved in a solution of 2.5:1 THF/DMF(0.60 mL) under N₂ atmosphere. After stirring at ambient temperature for3 hours, the reaction mixture was diluted with H₂O and the resultingprecipitate was filtered to give 27 mg (49%) of desired product.

Step H: Preparation of tert-butyl4-(2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperazine-1-carboxylate

2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yltrifluoromethane sulfonate (0.027 g, 0.0576 mmol), tert-butylpiperazine-1-carboxylate (0.0215 g, 0.115 mmol), Cs₂CO₃ (0.0282 g,0.0865 mmol), Pd₂ dba₃ (0.00528 g, 0.00576 mmol) and Binap-rac (0.00718g, 0.0115 mmol) were combined in toluene (0.5 mL) in a sealed vial. Thereaction mixture was stirred at 100° C. for 16 hours then concentratedunder reduced pressure. The resulting residue was diluted with EtOAc,washed with aqueous saturated NaHCO₃ and brine, dried over Na₂SO₄,filtered and concentrated under reduced pressure to an orange solid.Purification via flash column chromatography (40:1 DCM/MeOH followed by20:1 DCM/MeOH) provided 22 mg (76%) of desired product.

Step I: Preparation of2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)-8-(piperazin-1-yl)quinoline

To a solution of tert-butyl4-(2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperazine-1-carboxylate(0.022 g, 0.0436 mmol) in THF/MeOH (1 mL) was added 4 M HCl (0.109 mL,0.436 mmol) in dioxane. The reaction mixture was stirred at ambienttemperature for 16 hours then neutralized with 1 M NaOH and extractedwith EtOAc. The combined organic layers were washed with brine, driedover Na₂SO₄, filtered and concentrated under reduced pressure. Theresidue was purified via flash column chromatography (40:1 DCM/MeOHfollowed by 10:1 DCM/MeOH) to provide 4 mg (23%) of desired product as ayellow solid. MS APCI (+) m/z 405.4 (M+1) detected.

Example 2

2-(7-Methoxy-[1,2,4]triazolo[4,3-a]pyridin-3-yl)-8-(piperazin-1-yl)quinoline

Prepared as described in Example 1 using methanol in place of2-methoxyethanol in step A. MS APCI (+) m/z 361.3 (M+1) detected.

Example 3

1-(2-(7-Methoxy-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-amine

Prepared as described in Example 1 using 2-chloro-4-methoxypyridine inplace of 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting tert-butyl piperidin-4-ylcarbamate for tert-butylpiperazine-1-carboxylate in step H. MS APCI (+) m/z 375.2 (M+1)detected.

Example 4

4-(2-(7-Methoxy-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)morpholine

Prepared as described in Example 1 using 2-chloro-4-methoxypyridine inplace of 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting morpholine for tert-butyl piperazine-1-carboxylate in stepH. MS APCI (+) m/z 362.3 (M+1) detected.

Example 5

2-([1,2,4]-Triazolo[4,3-a]pyridin-3-yl)-8-(piperidin-1-yl)quinoline

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting piperidine for tert-butyl piperazine-1-carboxylate in stepH. MS ESI (+) m/z 330 (M+1) detected.

Example 6

2-([1,2,4]-Triazolo[4,3-a]pyridin-3-yl)-8-(piperazin-1-yl)quinoline

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B. MS ESI(+) m/z 331 (M+1) detected.

Example 7

(R)-2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)-8-(3-methylpiperazin-1-yl)quinoline

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting (R)-tert-butyl 2-methylpiperazine-1-carboxylate fortert-butyl piperazine-1-carboxylate in step H. MS ESI (+) m/z 345 (M+1)detected.

Example 8

2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)-8-((cis)-3,5-dimethylpiperazin-1-yl)quinoline

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting (cis)-tert-butyl 2,6-dimethylpiperazine-1-carboxylate fortert-butyl piperazine-1-carboxylate in step H. MS ESI (+) m/z 359 (M+1)detected.

Example 9

1-(2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-amine

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting tert-butyl piperidin-4-ylcarbamate for tert-butylpiperazine-1-carboxylate in step H. MS ESI (+) m/z 345 (M+1) detected.

Example 10

(1-(2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methanamine

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andsubstituting tert-butyl piperidin-4-ylmethylcarbamate for tert-butylpiperazine-1-carboxylate in step H. MS ESI (+) m/z 359 (M+1) detected.

Example 11

(3-(8(4-(Aminomethyl)piperidin-1-yl)quinolin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)methanolStep A: Preparation of 8-bromo-2-vinylquinoline

To 2,8-dibromoquinoline (1.836 g, 6.398 mmol) in THF/IPA (20 mL/10 mL)was added vinylborate (0.9428 g, 7.038 mmol) and triethylamine (0.7769g, 7.678 mmol). The reaction mixture was purged with N₂, and PdCl₂(dppf)dichloromethane adduct (0.3685 g, 0.4479 mmol) was added. The reactionwas stirred at 55° C. for 12 hours, then cooled to ambient temperature.The reaction mixture was diluted with EtOAc/H₂O, and the organic layerwas separated and concentrated to provide the crude product.

Step B: Preparation of 1-(8-bromoquinolin-2-yl)ethane-1,2-diol

To 8-bromo-2-vinylquinoline (0.886 g, 3.78 mmol) in acetone/H₂O (8 mL/2mL) was added 4-methylmorpholine N-oxide (1.06 g, 4.54 mmol) followed byOsO₄ (0.5 mL, 2.5% in t-BuOH). The reaction mixture was stirred for twohours at ambient temperature then diluted with dichloromethane. Theorganic layer was washed with saturated sodium sulfate and brine andconcentrated to give crude product.

Step C: Preparation of8-bromo-2-(2,2-dimethyl-1,3-dioxolan-4-yl)quinoline

To 1-(8-bromoquinolin-2-yl)ethane-1,2-diol (505 mg, 1.88 mmol) inacetone (5 mL) and 2,2-dimethoxypropane (5 mL, 1.88 mmol) was addedMontmorillonite K 10 (1 g). The reaction mixture was stirred for 2hours, then filtered and concentrated to give crude product.

Step D: Preparation of tert-butyl(1-(2-(2,2-dimethyl-1,3-dioxolan-4-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To 8-bromo-2-(2,2-dimethyl-1,3-dioxolan-4-yl)quinoline (101 mg, 0.328mmol) in toluene (1.3 mL) was added tert-butylpiperidin-4-ylmethylcarbamate (84.3 mg, 0.393 mmol), Pd(OAc)₂ (7.3 mg,0.033 mmol), Binap-rac (25 mg, 0.039 mmol) and Cs₂CO₃ (235 mg, 0.721mmol). The reaction was purged twice with nitrogen and heated at refluxovernight. The reaction mixture was cooled to ambient temperature andfiltered through Celite, and the solids were washed with EtOAc (20 mL).The filtrate was concentrated, and the crude material was purified bysilica gel chromatography (EtOAc/Hexane 1:6) provided final product.

Step E: Preparation of tert-butyl(1-(2-(1,2-dihydroxyethyl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl (1-(2-(2,2-dimethyl-1,3-dioxolan-4-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate (81 mg, 0.18 mmol)in THF (3 mL) was added HCl (20 mg, 0.55 mmol) (6M). The reaction wasstirred for 3 hours at ambient temperature and then diluted with EtOAc(10 mL) and saturated NaHCO₃ (5 mL). The phases were separated, and theorganic layer was dried and concentrated to give crude product.

Step F: Preparation of tert-butyl(1-(2-formylquinolin-8-yl)piperidin-4-yl)methylcarbamate

Sodium periodate (0.34 mL, 0.22 mmol was added drop-wise to a slurry ofsilica gel (0.4 g) in DCM (3 mL)). The reaction was stirred for 10minutes. tert-Butyl(1-(2-(1,2-dihydroxyethyl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(74 mg, 0.18 mmol) in DCM (2 mL) was then added to the slurry and thereaction was stirred for 30 minutes. The reaction was filtered and thecollected solids were washed with DCM (20 mL). The filtrate wasconcentrated to give the crude product.

Step G: Preparation of (E)-tert-butyl(1-(2-((2-(4-iodopyridin-2-yl)hydrazono)methyl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl (1-(2-formylquinolin-8-yl)piperidin-4-yl)methylcarbamate(112 mg, 0.303 mmol) in DCM (20 mL) was added1-(4-iodopyridin-2-yl)hydrazine (78.4 mg, 0.333 mmol). The reactionmixture was stirred for 1 hour and then taken on to directly to the nextstep.

Step H: Preparation of tert-butyl(1-(2-(7-iodo-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To (E)-tert-butyl(1-(2-((2-(4-iodopyridin-2-yl)hydrazono)methyl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(178 mg, 0.304 mmol) was added iodobenzene diacetate (IBD) (127 mg,0.395 mmol). The reaction was stirred for 2 hours. Additional IBD (0.5eq) was added and the reaction was stirred for another 2 hours and thenquenched with saturated Na₂S₂O₃ (5 mL). The organic layer was extractedwith DCM, dried (Na₂SO₄) and concentrated. The crude material waspurified by silica gel chromatography (EtOAc/Hexane/MeOH 2:1:0.1) toprovide the final product.

Step I: Preparation of tert-butyl(1-(2-(7-vinyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl(1-(2-(7-iodo-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(91 mg, 0.16 mmol) in THF/IPA (2/1 mL) was added C₂H₃BF₃K (31 mg, 0.23mmol), PdCl₂(dppf) dichloromethane adduct (13 mg, 0.016 mmol) andtriethylamine (24 mg, 0.23 mmol). The reaction was stirred for 2 hours,and then heated at 60° C. overnight. The reaction was cooled to ambienttemperature and concentrated. The crude material was purified by silicagel chromatography (20:1 Hexane/EtOAc) to provide the final product.

Step J: Preparation of tert-butyl(1-(2-(7-(1,2-dihydroxyethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl(1-(2-(7-vinyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(68 mg, 0.14 mmol) in acetone (2 mL) was added OsO₄ (178 mg, 0.014 mmol)(2% in tBuOH) and 4-methylmorpholine N-oxide (49 mg, 0.21 mmol) (50% inH₂O). The reaction was stirred for 2 hours and then concentrated. Thecrude material was purified by silica gel chromatography (20:1Hexane/EtOAc) to provide the final product.

Step K: Preparation of tert-butyl(1-(2-(7-formyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To a slurry of silica gel (0.1 g) in DCM (3 mL) was added sodiumperiodate (0.13 mL, 0.087 mmol). The reaction was stirred for 10minutes. tert-Butyl(1-(2-(7-(1,2-dihydroxyethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(30 mg, 0.058 mmol) in DCM (2 mL) was then added to the slurry. Thereaction was stirred for 30 minutes, then filtered and washed with DCM(20 mL). The organic layer was concentrated to give crude product.

Step L: Preparation oftert-butyl(1-(2-(7-(hydroxymethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl(1-(2-(7-formyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(10 mg, 0.021 mmol) in DCM/MeOH (1 mL/1 mL) was added NH₃ in IPA (0.1mL, 2M) and HOAc (0.2 mL), followed by NaB(OAc)₃H (13 mg, 0.062 mmol).The reaction was stirred for 5 hours and then concentrated. The crudematerial was purified by silica gel chromatography (DCM/MeOH/NH₄OH20:1:0.1) to provide the desired product (3 mg). MS ESI (+) m/z 489(M+1) detected.

Step M: Preparation of(3-(8-(4-(Aminomethyl)piperidin-1-yl)quinolin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)methanol

Prepared fromtert-butyl(1-(2-(7-(hydroxymethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamateaccording to the method of Example 1 (Step I). MS ESI (+) m/z 389 (M+1)detected.

Example 12

N-((3-(8-(4-(aminomethyl)piperidin-1-yl)quinolin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)methyl)ethanamineStep A: Preparation of tert-butyl(1-(2-(7-((ethylamino)methyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl(1-(2-(7-formyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(Example 11, steps A-K; 10 mg, 0.021 mmol) in DCM/MeOH (1 mL/1 mL) wasadded EtNH₂ (0.1 mL, 2M in THF) and HOAc (0.2 mL), followed byNaB(OAc)₃H (13 mg, 0.062 mmol). The reaction mixture was stirred for 5hours at ambient temperature and then concentrated. The crude materialwas purified by silica gel chromatography (DCM/MeOH/NH₄OH 20:1:0.1) toprovide the desired product (4 mg). MS APCI (+) m/z 343 (M+1) detected.

Step B: Preparation ofN-((3-(8-(4-(aminomethyl)piperidin-1-yl)quinolin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)methyl)ethanamine

Prepared from tert-butyl(1-(2-(7-((ethylamino)methyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamateas described in Example 1 (step I). MS ESI (+) m/z 416 (M+1) detected.

Example 13

(1-(2-(7-(Aminomethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methanamine

Prepared as described in Example 12 using NH₃ in replacement of EtNH₂.MS ESI (+) m/z 388 (M+1) detected.

Example 14

1-(3-(8-(4-(Aminomethyl)piperidin-1-yl)quinolin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)ethane-1,2-diolStep A: Preparation of tert-butyl(1-(2-formylquinolin-8-yl)piperidin-4-yl)methylcarbamate

Prepared as described in Example 1, Step H, substituting8-bromoquinoline-2-carbaldehyde for2-(7-(2-methoxyethoxy)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yltrifluoromethane sulfonate, and tert-butyl piperidin-4-ylmethylcarbamatefor tert-butyl piperazine-1-carboxylate.

Step B: Preparation of tert-butyl(1-(2-((2-(4-iodopyridin-2-yl)hydrazono)methyl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

Prepared as described in Example 1, steps D-E, using tert-butyl(1-(2-formylquinolin-8-yl)piperidin-4-yl)methylcarbamate in place of8-(tert-butyldimethylsilyloxy)quinoline-2-carbaldehyde, and2-hydrazinyl-4-iodopyridine in place of2-Hydrazinyl-4-(2-methoxyethoxy)pyridine.

Step C: Preparation of tert-butyl(1-(2-(7-vinyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl(1-(2-(7-iodo-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(91 mg, 0.16 mmol) in THF/IPA (2/1 mL) was added C₂H₃BF₃K (31 mg, 0.23mmol), PdCl₂(dppf) dichloromethane adduct (13 mg, 0.016 mmol) andtriethylamine (24 mg, 0.23 mmol). The reaction was stirred for 2 hours,followed by heating at 60° C. overnight. The reaction was then cooled toambient temperature and concentrated, and the residue was purified byflash column chromatography (20:1 Hexane/EtOAc) providing the desiredproduct (90% yield; 0.068 g).

Step D: Preparation of tert-butyl(1-(2-(7-(1,2-dihydroxyethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate

To tert-butyl(1-(2-(7-vinyl-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(68 mg, 0.14 mmol) in acetone (2 mL) was added OsO₄ (178 mg, 0.014 mmol)(2% in tBuOH) and NMO (49 mg, 0.21 mmol) (50% in H₂O). The reaction wasthen stirred for 2 hours, then concentrated and purified by flash columnchromatography (20:1 Hexane/EtOAc), affording the desired product.

Step D: Preparation of1-(3-(8-(4-(Aminomethyl)piperidin-1-yl)quinolin-2-yl)-[1,2,4]triazolo[4,3-a]pyridin-7-yl)ethane-1,2-diol

To tert-butyl(1-(2-(7-(1,2-dihydroxyethyl)-[1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-4-yl)methylcarbamate(2 mg, 0.004 mmol) in DCM (1 mL) was added TFA (0.5 mL). The reactionwas then stirred for 1 hour, then concentrated and purified by flashcolumn chromatography (DCM/MeOH/NH₄OH 20:1:0.1), affording the finalproduct as a white solid (0.002 g; quantitative yield). MS ESI (+) m/z419 (M+1) detected.

Example 15

(1-(2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-yl)methanol

Prepared as described in Example 1 using 2-chloropyridine as areplacement for 2-chloro-4-(2-methoxyethoxy)pyridine in step B, andpiperidin-3-ylmethanol for tert-butyl piperazine-1-carboxylate in stepH. MS ESI (+) m/z 360.3 (M+1) detected.

Example 16

(1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-6-fluoroquinolin-8-yl)piperidin-4-yl)methanamineStep A: Preparation of 8-bromo-6-fluoro-2-methylquinoline

2-Bromo-4-fluorobenzenamine (10 g, 52.6 mmol) was weighed into a roundbottom flask, and dissolved in 40 ml, of 6N HCl. The reaction mixturewas then heated to reflux, followed by drop-wise addition of(E)-but-2-enal (4.578 mL, 55.3 mmol) mixed with 1.0 mL deionized waterover 25 minutes. Following complete addition the reaction was heated at100° C. for an additional 35 minutes, until all the2-bromo-4-fluorobenzenamine had been consumed. The reaction was cooledto ambient temperature, followed by addition of 50 mL of Et₂O. Thereaction was stirred for 5 minutes followed by removal of Et₂O bypartitioning. The aqueous layer was replaced into the original reactionflask and ZnCl₂ (3.586 g, 26.3 mmol) was then added in two portionsfollowed by cooling to 0° C. over 30 minutes. The pH of the crudereaction mixture was then adjusted to pH=8.0 using concentrated NH₄OH.The crude mixture was then extracted with Et₂O, followed by ethylacetate. The combined organics were then dried over Na₂SO₄, and thenconcentrated in vacuo, affording the desired product as a brown solid(10.7 g, 85% yield). MS APCI (+) m/z 240.2 and 242.2 (M+1 of each Brisotope) detected.

Step B: Preparation of 8-bromo-2-(dibromomethyl)-6-fluoroquinoline

8-Bromo-6-fluoro-2-methylquinoline (10.7 g, 44.6 mmol) was weighed intoa 1 neck flask, followed by addition of NaOAc (21.9 g, 267 mmol). Thesolids were suspended in 500 mL of AcOH, and the reaction heated to 70°C. Bromine (6.85 mL, 134 mmol) was the added drop-wise over 25 minutesas a solution in 30 mL of AcOH. Following complete addition, thereaction was stirred at 100° C. for 1 hour. The reaction was then cooledto ambient temperature, followed by pouring onto 750 cc of ice. The icewas allowed to melt completely and the brown slurry separated bypartitioning 4×400 mL ethyl acetate. The combined organics were thendried over MgSO₄, and concentrated in vacuo to afford a brown solid(17.2 g, 97% yield).

Step C: Preparation of ethyl 8-bromo-6-fluoroquinoline-2-carboxylate and8-bromo-6-fluoroquinoline-2-carboxylic acid

8-Bromo-2-(dibromomethyl)-6-fluoroquinoline (17.2 g, 43.2 mmol) wasweighed into a flask and dissolved in 250 mL of EtOH, followed byaddition of silver nitrate (23.5 g, 138 mmol) in 100 mL of 1:1 EtOH/H₂O.The reaction was heated to reflux for 1 hour, at which time all startingmaterial had been consumed. The reaction was removed from heat andfiltered hot through a medium frit scintered glass funnel, affording5.84 g of 8-bromo-6-fluoroquinoline-2-carboxylic acid as a white/yellowpowder. The mother liquor was concentrated in vacuo, followed byextractive work-up (200 mL ethyl acetate/water). The combined organicswere dried over Na₂SO₄ and concentrated in vacuo to afford the desiredproducts as an orange-brown semi solid (99% overall; 6.4 g and 5.8 grespectively). MS APCI (+) m/z 298 and 300 (M+1 of each isotope)detected; MS APCI (−) m/z 268 and 269.9 (M−1 of each Br isotope)detected.

Step D: Preparation of (8-bromo-6-fluoroquinolin-2-yl)methanol

Ethyl 8-bromo-6-fluoroquinoline-2-carboxylate (3.201 g, 10.7 mmol) wasweighed into a flask, and dissolved in 100 mL of DCM. The reaction wascooled to −78° C., followed by drop-wise addition of DIBAL-H (21.48 mL,32.22 mmol) over 10 minutes. The reaction was then allowed to stir andwarm to ambient temperature over 2 hours at which time the startingmaterial had been consumed. The reaction was quenched with 10 mL MeOH,followed by addition of 100 mL of Rochelle's Salts, and stirredovernight to remove the emulsion. The reaction was then partitioned withethyl acetate. The combined organic fractions were concentrated invacuo. The crude semi solid was purified by flash column chromatography(eluting with a 20-50% ethyl acetate/hexanes gradient), affording thedesired product as an orange-yellow semi solid (2.27 g, 42% yield) MSAPCI (+) m/z 256.1 and 258 (M+1 of each Br isotope) detected.

Step E: Preparation of 8-bromo-6-fluoroquinoline-2-carbaldehyde

(8-bromo-6-fluoroquinolin-2-yl)methanol (2 g, 7.8 mmol), DMSO (8.9 mL,125.0 mmol), and TEA (4.9 mL, 35 mmol) were weighed into a flask anddissolved in a 10 mL of DCM, followed by cooling to 0° C. Pyridiniumsulfate (4.351 g, 27.3 mmol) was added and the reaction stirred 0° C.for 1 hour. The reaction was poured onto 50 mL water and extracted withethyl acetate. The combined organics were then dried over MgSO₄, thenconcentrated in vacuo affording a yellow/white semi-solid, which wasfurther purified by triturating with 20% ethyl acetate/Hexanes,affording the desired product as a tan solid (1.35 g, 68% yield).

Step F: Preparation of(E)-1-((8-bromo-6-fluoroquinolin-2-yl)methylene)-2-(pyridin-2-yl)hydrazine

8-Bromo-6-fluoroquinoline-2-carbaldehyde (100 mg, 0.39 mmol) and1-(pyridin-2-yl)hydrazine (43 mg, 0.39 mmol) were dissolved in 15 mL ofabsolute EtOH, and heated to reflux for 2 hours, at which time allstarting material had been consumed. The reaction was then cooled toambient temperature, and the yellow/orange product was collected byfiltration (wash EtOH), affording (100 mg, 73.6% yield) as a yellowsolid. MS APCI (+) m/z 345.1 and 347 (M+1 of each Br isotope) detected.

Step G: Preparation of2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-8-bromo-6-fluoroquinoline

(E)-1-((8-Bromo-6-fluoroquinolin-2-yl)methylene)-2-(pyridin-2-yl)hydrazine(100 mg, 0.290 mmol) was weighed into a 25 mL 1 neck round bottom flaskand suspended in 6.0 mL of DCM, followed by addition of iodobenzenediacetate (103 mg, 0.32 mmol). The reaction was stirred at 23° C.overnight, after which all starting materials had been consumed. Thereaction was transferred to a reparatory funnel and partitioned between30 mL DCM and 30 mL Na₂SO₃, and the aqueous layer was washed with DCM.The combined organics were dried over magnesium sulfate and concentratedin vacuo to afford a white/yellow powder. The powder was triturated with10 mL of anhydrous Et₂O, affording the desired product as a white solid(74 mg, 74.4% yield). MS APCI (+) m/z 343 (M+1) detected.

Step H: Preparation of tert-butyl(1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-6-fluoroquinolin-8-yl)piperidin-4-yl)methylcarbamate

2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)-8-bromo-6-fluoroquinoline (70 mg,0.20 mmol), tert-butyl piperidin-4-ylmethylcarbamate (56.8 mg, 0.27mmol), and Cs₂CO₃ (66 mg, 0.20 mmol) were weighed into a 5.0 mL reactionvial and suspended in 2.0 mL of anhydrous toluene. The solution waspurged with Argon followed by addition of Pd₂ dba₃ (93.4 mg, 0.10 mmol),Binap-rac (12.7 mg, 0.020 mmol), then heated to 95° C. for 48 hours. Thereaction was cooled to ambient temperature, followed by filtrationthrough a celite plug to remove catalyst. The crude reaction wasconcentrated in vacuo and used without further purification (97 mg, 97%yield). MS APCI (+) m/z 477 (M+1) detected.

Step I: Preparation of(1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-6-fluoroquinolin-8-yl)piperidin-4-yl)methanaminebis(trifluoroacetate)

tert-Butyl (1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-6-fluoroquinolin-8-yl)piperidin-4-yl)methylcarbamate (97 mg, 0.204 mmol) wasweighed into a flask and dissolved in 10 mL of anhydrous DCM. TFA (314μL, 4.07 mmol) was added and the reaction was stirred at ambienttemperature for 1 hour. The reaction was concentrated in vacuo, followedby trituration with anhydrous Et₂O, affording the desired product (43mg, 0.114 mmol, 56.1% yield) as a yellow solid. APCI (+) m/z 377 (M+1)detected.

Example 17

(1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-5-fluoroquinolin-8-yl)piperidin-4-yl)methanamine

Prepared as described in Example 16 using 2-bromo-5-fluoro-aniline inplace of 2-bromo-4-fluoroaniline. APCI (+) m/z 377 (M+1) detected.

Example 18

(1-(2-([1,2,4]-Triazolo[4,3-a]pyridin-3-yl)-6-chloroquinolin-8-yl)piperidin-4-yl)methanamine

Prepared as previously described in Example 16 using2-bromo-4-chloroaniline in place of 2-bromo-4-fluoro aniline. MS APCI(+) m/z 393.1 and 395.1 (M+1 of each isotope) detected.

Example 19

2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-8-(3-methylpiperidin-1-yl)quinoline

2-([1,2,4]Triazolo[4,3-a]pyridin-3-yl)quinolin-8-yltrifluoromethanesulfonate (0.040 g, 0.10 mmol), 3-methylpiperidine(0.016 mL, 0.13 mmol), cesium carbonate (0.050 g, 0.15 mmol), Pd₂ dba₃(0.005 g, 0.005 mmol) and 2,2′-bis(diphenylphosphino)-1,1′-binaphthyl(0.006 g, 0.01 mmol) were added to minimal degassed toluene and heatedto 92° C. overnight in a sealed tube. After stirring overnight, DCM wasadded, and solids removed through filtration (Waters filter), followedby concentration in vacuo. The crude mixture was then purified by flashcolumn chromatography (gradient elution of 6% NH₄ in MeOH/DCM). Thedesired product was isolated, along with triflate starting material. Themixture was then treated with 3 N HCl aqueous at 95° C. overnight. Thereaction was then cooled and solids removed by filtration. The filtratewas purified by flash column chromatography (gradient elution of 6% NH₄in MeOH/DCM (Rf ˜0.1 in 6% NH₄ in MeOH (5%) and DCM (95%)). The desiredproduct was isolated as a yellow solid, 0.005 g (15% yield). MS ESI (+)m/z 344.3 (M+1) detected.

Example 20

1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-ol

Prepared as described in Example 19 using piperidin-3-ol in place of3-methylpiperidine. MS ESI (+) m/z 346.2 (M+1) detected.

Example 21

(R)-1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-amineStep A: Preparation of (R)-tert-butyl1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-ylcarbamate

2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yltrifluoromethanesulfonate (0.040 g, 0.10 mmol), (R)-tert-butylpiperidin-3-ylcarbamate (0.026 g, 0.13 mmol), cesium carbonate (0.050 g,0.15 mmol), Pd₂ dba₃ (0.0046 g, 0.0051 mmol) and2,2′-bis(diphenylphosphino)-1,1′-binaphthyl (0.0063 g, 0.010 mmol) wereadded to minimal degassed toluene and heated in a seal vial overnight at92° C. After stirring overnight, DCM was added, solids removed throughfiltration (Waters filter), and the filtrate was concentrated. The crudematerial was purified by flash column chromatography (gradient elutionof 6% NH₄ in MeOH/DCM). The desired product (30 mg) was isolated, as a1:1 mixture with the triflate starting material. The mixture was usedwithout further purification.

Step B: Preparation of(R)-1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-amine

(R)-tert-butyl1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-ylcarbamate(0.030 g, 0.067 mmol) was added to a 1:1 TFA-DCM and stirred for about 2hours, at which time the reaction appeared complete by LC/TLC. The crudemixture was evaporated and purified by flash column chromatography(Horizon, using a gradient elution of 5% NH₄ in MeOH/DCM). The desiredproduct was isolated as a light yellow solid (10 mg; 43% yield). MS ESI(+) m/z 345.2 (M+1) detected.

Example 22

(S)-1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperidin-3-amine

Prepared as described in Example 21 using (S)-tert-butylpiperidin-3-ylcarbamate in place of (R)-tert-butylpiperidin-3-ylcarbamate. MS ESI (+) m/z 345.2 (M+1) detected.

Example 23

2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-8-(3,3-dimethylpiperazin-1-yl)quinoline

Prepared as described in Example 21 using tert-butyl2,2-dimethylpiperazine-1-carboxylate in place of (R)-tert-butylpiperidin-3-ylcarbamate. MS ESI (+) m/z 359.2 (M+1) detected.

Example 24

4-((4-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)piperazin-1-yl)methyl)-2-methylthiazole

Prepared as described in Example 1 using2-methyl-4-(piperazin-1-ylmethyl)thiazole in place of tert-butyl4-aminopiperidine-1-carboxylate in step H. MS ESI (+) m/z 442.3 (M+1)detected.

Example 25

2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-7-bromo-8-(3-methylpiperidin-1-yl)quinolineStep A: Preparation of 7-bromo-2-methylquinolin-8-ol

To a 250 mL flask was added toluene (150 mL) and t-BuNH₂ (7.26 mL, 69.1mmol). The solution was cooled to −25° C. and bromine (1.95 mL, 38.0mmol) was added. The solution was cooled to −78° C. and2-methylquinolin-8-ol (5.5 g, 34.6 mmol) was added as a CH₂Cl₂ solution(15 mL). The reaction mixture was then gradually warmed to ambienttemperature over 6 hours. The mixture was washed with water (50 mL) andthen treated with 3.0 M aqueous NaOH (250 mL). This provided copiousamounts of precipitate, which went into solution after about 600 mLwater was added. The layers were mixed and separated. The alkalineextract was carefully acidified with concentrated HCl (about 50 mL). Thesolution was extracted with CH₂Cl₂ (4×200 mL), and the combined extractswere washed with brine and dried over Na₂SO₄, filtered and concentrated.The original water wash was found to contain a significant amount ofproduct, so 10 mL 1M HCl was added and the acidic solution was extractedwith CH₂Cl₂ (2×75 mL) and these layers were also washed with brine,dried over Na₂SO₄, filtered and concentrated. The organic phases werecombined to provide 5.0 g (60%) of the desired product as a red/brownsolid.

Step B: Preparation of7-bromo-8-(tert-butyldimethylsilyloxy)-2-methylquinoline

To the product from Step A (3.0 g, 12.6 mmol) was added imidazole (1.89g, 27.7 mmol) and CH₂Cl₂ (40 mL). The solution was cooled to 0° C. andthen tert-butylchlorodimethylsilane (2.09 g, 13.9 mmol) was added in oneportion. The reaction was gradually warmed to ambient temperature over 1hour and then stirred overnight. The mixture was diluted with asaturated aqueous NH₄Cl solution (25 mL) and CH₂Cl₂ (40 mL). The layerswere mixed and separated and the organic layer was washed with brine anddried over Na₂SO₄, filtered and concentrated. The crude product waspurified by column chromatography (2 to 20% CH₂Cl₂/hexanes) to provide3.36 g (76%) of the desired product as a white solid.

Step C: Preparation of7-bromo-8-(tert-butyldimethylsilyloxy)quinoline-2-carbaldehyde

A slurry of SeO₂ (0.869 g, 7.83 mmol) and 1,4-dioxane (20 mL) was warmedto 80° C. and then the product from Step B (2.3 g, 6.53 mmol) was addedas a 1,4-dioxane solution (20 mL). The mixture was stirred at 80° C. for32 hours. The mixture was cooled to ambient temperature and filteredthrough GF/F filter paper and the residual solid was washed with CH₂Cl₂.The filtrate was concentrated and purified by passing through a silicagel plug, eluting with 50% CH₂Cl₂/hexane to provide 2.14 g (89%) of theproduct as a yellow/orange solid.

Step D: Preparation of7-bromo-8-(tert-butyldimethylsilyloxy)-2-((2-(pyridin-2-yl)hydrazono)methyl)quinoline

To the product from Step C (2.95 g, 8.05 mmol) was added EtOH (30 mL,anhydrous). To this solution was added 2-hydrazinylpyridine (0.967 g,8.86 mmol). The mixture was stirred at ambient temperature for 24 hours.The resulting precipitate was isolated by vacuum filtration and washedwith cold EtOH and then dried in vacuo to afford 2.98 g (73%) of thedesired product.

Step E: Preparation of2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-7-bromo-8-(tert-butyldimethylsilyloxy)quinoline

To the product from Step D (2.95 g, 6.45 mmol) was added CH₂Cl₂ (60 mL).Iodobenzene diacetate (2.28 g, 7.09 mmol) was added and the mixturestirred at ambient temperature overnight. The mixture was concentratedand the product purified directly by column chromatography (1 to 8%MeOH/CH₂Cl₂) to afford 2.87 g (88%) of the product.

Step F

Preparation of2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-7-bromoquinolin-8-ol: To theproduct from Step E (2.8 g, 6.15 mmol) was added THF (60 mL). Thesolution was cooled to 0° C. then TBAF.3H₂O (2.33 g, 7.38 mmol) wasadded and the mixture was stirred for 1 hour. The mixture was thendiluted with EtOAc (100 mL) and then washed with saturated aqueousNaHCO₃ (75 mL). The layers were separated and the aqueous phase washedwith EtOAc (100 mL). The combined organic phases were washed with brineand dried over Na₂SO₄, filtered and concentrated. The crude mixture wastriturated with MeOH and the mixture was filtered and the solid waswashed with Et₂O (0.730 g; 35%).

Step G: Preparation of 2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-7-bromoquinolin-8-yl trifluoromethanesulfonate

The product from Step F (0.730 g, 2.14 mmol) was suspended in THF (6 mL)and DMF (2 mL). NEt₃ (0.746 mL, 5.35 mmol) was added followed byN-Phenyltriflimide (0.917 g, 2.57 mmol). The mixture was stirred atambient temperature overnight. There was still starting materialpresent, so additional N-Phenyltriflimide (0.300 g) was added and thereaction stirred for an additional 1.0 hours. The mixture was dilutedwith water (25 mL), stirred for 30 min and then filtered. The solidswere washed with water, Et₂O (10 mL) and then hexanes. The solid wasdried in vacuo until constant weight 0.890 g (88%) and was used directlyin the next step.

Step H: Preparation of2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)-7-bromo-8-(3-methylpiperidin-1-yl)quinoline

The product from Step G (0.025 g, 0.0528 mmol) and 3-methylpiperidine(0.0186 mL, 0.158 mmol) were added to a small microwave reaction vialfollowed by NMP (0.250 mL). The vessel was placed inside the center ofthe microwave oven and then it was exposed to microwave irradiation (250W) for 20 minutes at a temperature of 195° C. After the irradiation, thereaction mixture was cooled to ambient temperature, poured into 10 mL ofwater and extracted with CH₂Cl₂ (3×10 mL). The combined organic extractswere washed with saturated brine and dried over anhydrous Na₂SO₄. Afterremoval of the solvent, the residue was purified by columnchromatography on silica gel to give the product as a dark orangesemisolid which was ˜80% pure. This material was purified viaPreparative TLC using 50% Acetone/hexane as eluent to provide 0.005 g(22%) of the product as an orange film. MS ESI (+) m/z 422.3 (M)detected.

Example 26

1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)-4-methylpiperidin-4-amineStep A: Preparation of benzyl1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)-4-methylpiperidin-4-ylcarbamate

Prepared as described in Example 1 using benzyl4-methylpiperidin-4-ylcarbamate in place of tert-butyl4-aminopiperidine-1-carboxylate in step H.

Step B: Preparation of1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)-4-methylpiperidin-4-amine

To the product from Step A (0.115 g, 0.233 mmol) was added HCl (1.17 mL,7.00 mmol, 6.0N aqueous). The mixture was stirred at 90° C. for 45minutes. The mixture was then cooled to ambient temperature and dilutedwith water (10 mL) and with CH₂Cl₂ (10 mL). The layers were mixed andseparated and the aqueous phase was washed once more with CH₂Cl₂ (10 mL)and was then treated with saturated aqueous Na₂CO₃ until pH=10. Theaqueous phase was then extracted with CH₂Cl₂ (3×10 mL). The combinedorganic phases were washed with water followed by brine and then driedover Na₂SO₄, filtered and concentrated to provide 0.058 g (66%) of theproduct >96% pure by HPLC. MS ESI (+) m/z 359.1 (M+1) detected.

Example 27

(cis)-1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)-3-fluoropiperidin-4-amineStep A: Preparation of tert-butyl4-(trimethylsilyloxy)-5,6-dihydropyridine-1(2H)-carboxylate

To a 1.0 L flask was added tert-butyl 4-oxopiperidine-1-carboxylate(150.0 g, 752.8 mmol), which was dissolved in DMF (400 mL). To thissolution was added TMS-Cl (114.7 mL, 903.4 mmol), followed by NEt₃(251.8 mL, 1807 mmol). The resulting heterogeneous mixture was warmed to70° C. and stirred overnight under a N₂ atmosphere. The mixture wascooled to ambient temperature, diluted with hexanes (250 mL) andfiltered. The solids were washed with hexanes (4×250 mL). The combinedorganic phases were washed with a saturated aqueous NaHCO₃ (3×250 mL)and brine (3×250 mL), dried over Na₂SO₄, and concentrated. The crudeproduct was carried on directly to the next step.

Step B: Preparation of tert-butyl3-fluoro-4,4-dihydroxypiperidine-1-carboxylate

To the product from Step A (204 g, 750 mmol) was added CH₃CN (1500 mL).To this solution was added Selectfluor (292 g, 825 mmol) portionwise (25grams every 5 minutes) while cooling the reaction mixture in a waterbath. The reaction was stirred for 18 hours at ambient temperature. Themixture was concentrated to dryness and the residue dissolved in EtOAc(750 mL) and brine (500 mL). The organic layer was washed with brine(250 mL) and dried over Na₂SO₄, filtered and concentrated to dryness.The crude product was dissolved in minimal EtOAc (150 mL) with heatinguntil the solution was homogeneous and the solution was allowed to coolto ambient temperature. Hexane (100 mL) was added until the solution hadbecome cloudy white. The mixture was allowed to sit undisturbed forabout 12 hours and the resulting solid isolated by filtration. Thisprovided 99 g (56%) of the product as a white solid.

Step C: Preparation of tert-butyl4-(benzylamino)-3-fluoropiperidine-1-carboxylate

To a slurry of NaBH₄ (7.567 g, 200.0 mmol) in DCE (200 mL) was added2-Ethylhexanoic acid (95.50 mL, 600.0 mmol) slowly over 30 minutes viaaddition funnel. The mixture was stirred at ambient temperature for 4hours with venting to release H₂. In a separate 1 L flask was added theproduct from Step B (23.53 g, 100 mmol), benzylamine (16.37 mL, 150.0mmol) and DCE (400 mL). The hydride solution was then added via additionfunnel to the mixture over 1 hours while cooling the reaction in a waterbath. The reaction was stirred at ambient temperature for 2 days, thendiluted with water (100 mL) and concentrated in vacuo to remove solvent.The residue was partitioned between EtOAc (300 mL) and a saturatedaqueous Na₂CO₃ solution (2×75 mL). The mixture was shaken, the layersseparated and the organic phase washed again with a saturated aqueousNa₂CO₃ solution (100 mL) and finally with brine (50 mL). The aqueousphases were extracted with CHCl₃ (3×75 mL), and the organic phases weredried over Na₂CO₃, filtered and concentrated. The crude product waspurified by column chromatography (EtOAc/Hexane) providing 19.55 g ofthe pure cis product and 5.0 g of a cis/trans mixture (80%).

Step D: Preparation of cis-tent-butyl4-amino-3-fluoropiperidine-1-carboxylate

To a 250 mL glass Parr vessel was added the product from Step C (14.8 g,48.0 mmol) and EtOH (100 mL). Pearlman's Catalyst (4.72 g, 3.36 mmol)was added and the mixture was shaken in a Parr reactor under 40-45 psiH₂ for 15 hours. The reaction was filtered through celite and the celitewas washed with EtOAc. The filtrate was concentrated and the residue wasdissolved in CH₂Cl₂ and filtered through celite, and the celite waswashed with CH₂Cl₂ and EtOAc. The filtrate was concentrated to afford10.2 g (92%) of the desired product as a thick oil that slowlysolidified to a white solid.

Step E: Preparation of cis-tent-butyl4-(benzyloxycarbonylamino)-3-fluoropiperidine-1-carboxylate

To a solution of the product from Step D (10 g, 45.8 mmol) in THF (90mL) and water (20 mL) was added K₂CO₃ (8.23 g, 59.6 mmol). Once thesolid was dissolved benzyl chloroformate (7.19 mL, 50.4 mmol) was added.The reaction was stirred vigorously at ambient temperature for 5.0hours. Once the reaction was complete by TLC the reaction was dilutedwith EtOAc (100 mL) and water (20 mL). The layers were separated and theorganic layer was washed with brine, then dried over Na₂SO₄, filteredand concentrated in vacuo. The product was purified by columnchromatography (EtOAc/hexane) to afford 12.9 g (80%) of a sticky, whitefoam.

Step F: Preparation of cis-benzyl-3-fluoropiperidin-4-ylcarbamate

(cis)-tert-Butyl4-(benzyloxycarbonylamino)-3-fluoropiperidine-1-carboxylate (7.5 g, 21.3mmol) was weighed into a 500 mL 1 neck round bottom and dissolved in 200mL of DCM, followed by addition of TFA (16.4 mL, 213 mmol) and stirringat ambient temperature for 1 hour, at which time all bubbling had ceasedand the reaction appeared complete by TLC. The crude reaction wasconcentrated in vacuo, followed by aqueous work-up with 2 N NaOH andDCM. The combined organics were dried over Na₂SO₄, filtered andconcentrated in vacuo to afford benzyl(cis)-3-fluoropiperidin-4-ylcarbamate (4.25 g, 16.8 mmol, 79.2% yield)as a white solid.

Step G: Preparation of cis-benzyl1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)-3-fluoropiperidin-4-ylcarbamate

Prepared as described in Example 1 usingcis-benzyl-3-fluoropiperidin-4-ylcarbamate in place of tent-butyl4-aminopiperidine-1-carboxylate in step H.

Step H: Preparation of1-(2-([1,2,4]triazolo[4,3-a]pyridin-3-yl)quinolin-8-yl)-3-fluoropiperidin-4-amine

To the product from Step G (0.185 g, 0.373 mmol) was added HCl (1.86 mL,11.2 mmol, 6.0M aqueous) and the solution was warmed to 90° C. andstirred for 0.5 hours. The mixture was cooled to ambient temperature anddiluted with water (10 mL) and with CH₂Cl₂ (10 mL). The layers weremixed and separated and the aqueous phase was treated with saturatedaqueous Na₂CO₃ until pH=10. The aqueous phase was extracted with CH₂Cl₂(4×10 mL). The combined organic phase was washed with water and brine,dried over Na₂SO₄, filtered and concentrated. The crude residue waspurified by column chromatography (1 to 10% MeOH w/6% NH₄OH/CH₂Cl₂) toafford 0.094 g (69%) of the desired product as a pale yellow/orangesolid. MS ESI (+) m/z 363.2 (M+1) detected.

What is claimed is:
 1. A compound of general Formula I:

or a pharmaceutically acceptable salt thereof, wherein: R¹⁰ and R¹¹together with the N to which they are attached form a 4-8 memberedheterocyclic ring optionally having an additional ring heteroatomselected from N and O, wherein the heterocyclic ring is optionallysubstituted with one or more R⁹ groups; each R⁹ is independentlyselected from halogen, (1-6C)alkyl, NR^(f)R^(g), -(1-6Calkyl)NR^(h)R^(i), OR^(j), (1-6C alkyl)OR^(k), C(O)NR^(m)R^(n),C(O)O(1-6C alkyl), and -(1-6C alkyl)NR^(h)C(O)O(1-6C alkyl); B is H,OR^(a), (1-6C alkyl)NR^(b)R^(c), (1-6C alkyl)OH, NR^(b)R^(c), orCH(OH)CH₂OH; R¹ is H, F, Cl, Br, methyl, ethyl, cyclopropyl or CN;R^(1a), R², R³ and R⁴ are independently H, F, Cl, Br, methyl, ethyl,cyclopropyl or CN; R⁵ and R⁷ are independently H, F, Me or CN; R⁶ is H,F, Me, Br, CN, cyclopropyl or phenyl; R^(a) is H, (1-6C alkyl), -(1-6Calkyl)-O-(1-6C alkyl) or -(1-6C alkyl)-O-(3-6C cycloalkyl); each R^(b),R^(c), R^(d), R^(e), R^(f), R^(g), R^(h), R^(i), R^(j), R^(k), and R^(m)is independently selected from H and (1-6C alkyl); and R^(n) is H, (1-6Calkyl) or O-(1-6C alkyl).
 2. A compound of claim 1, wherein NR¹⁰R¹¹forms a heterocyclic ring selected from piperidinyl, piperazinyl, andmorpholinyl, each of which is unsubstituted or substituted with one ormore R⁹ groups.
 3. A compound of claim 1, wherein each R⁹ isindependently selected from Me, NH₂, CH₂NH₂, OH, CH₂OH, C(O)OMe, C(O)NH₂and CH₂NHCO₂t-Bu.
 4. A compound of claim 1, wherein B is H.
 5. Acompound of claim 1, wherein B is OR^(a).
 6. A compound of claim 5,wherein B is selected from OMe and —OCH₂CH₂OMe.
 7. A compound of claim1, wherein B is (1-6C alkyl)NR^(b)R^(c).
 8. A compound of claim 7,wherein B is CH₂NHEt or CH₂H₂.
 9. A compound of claim 1, wherein B is(1-6C alkyl)OH.
 10. A compound of claim 9, wherein B is CH₂OH.
 11. Acompound of claim 1, wherein B is CH(OH)CH₂OH.
 12. A compound of claim1, wherein R^(1a) is H, F or Cl.
 13. A compound of claim 1, wherein R²is H or F.
 14. A compound of claim 1, wherein R³ and R⁴ are H.
 15. Acompound of claim 1, wherein R⁵, R⁶ and R⁷ are H.
 16. A pharmaceuticalcomposition, which comprises a compound of Formula I of claim 1, or apharmaceutically acceptable salt thereof, and a pharmaceuticallyacceptable diluent or carrier.
 17. A process for the preparation acompound of claim 1, which comprises: (a) coupling a correspondingcompound having the formula II

wherein L¹ represents a leaving atom or group, with a compound havingthe formula HNR¹⁰R¹¹ wherein NR¹⁰R¹¹ represents a 4-8 memberedheterocyclic ring optionally having an additional heteroatom selectedfrom N and O and optionally substituted with one or more R⁹ groups,using a palladium catalyst and a ligand in the presence of a base; or(b) reacting a compound of Formula III

with a compound having the Formula IV

in the presence of an organo hypervalent iodine reagent; or (c) for acompound of Formula I where B is Or^(a), reacting a correspondingcompound having the Formula V

with a compound of the formula R^(a)-L², wherein L² represents a leavingatom or group, in the presence of a base; (d) for a compound of FormulaI wherein B is (CH₂)NR^(b)R^(c), reacting a corresponding compoundhaving the Formula VI

with hydrazine; and removing any protecting group or groups and, ifdesired, forming a salt.